Issue 43, 2014
From the journal:

Chemical Communications

Sequential one-pot enzymatic synthesis of oligo-N-acetyllactosamine and its multi-sialylated extensions†

Wei-Ting Chien,a   Chien-Fu Liang,a   Ching-Ching Yu,a   Chien-Hung Lin,a   Si-Peng Li,a   Indah Primadona,ab   Yu-Ju Chen,bc   Kwok Kong T. Mongd  and  Chun-Cheng Lin*ac  

* Corresponding authors

a Department of Chemistry, National Tsing Hua University, 101, Sec. 2, Kuang Fu Rd., Hsinchu, Taiwan
E-mail: cclin66@mx.nthu.edu.tw
Fax: +886 3 5711082
Tel: +886 3 5753147

b Institute of Chemistry, Academia Sinica, 128, Sec. 2, Academia Rd., Nankang, Taipei 11529, Taiwan

c Genomic Research Center, Academia Sinica, 128, Sec. 2, Academia Rd., Nankang, Taipei 11529, Taiwan

d Applied Chemistry Department, National Chiao Tung University, 1001, University Road, Hsinchu 30010, Taiwan

Abstract

A simple and efficient protocol for the preparative-scale synthesis of various lengths of oligo-N-acetyllactosamine (oligo-LacNAc) and its multi-sialylated extensions is described. The strategy utilizes one thermophilic bacterial thymidylyltransferase (RmlA) coupled with corresponding sugar-1-phosphate kinases to generate two uridine diphosphate sugars, UDP-galactose and UDP-N-acetylglucosamine. By incorporating glycosyltransferases, oligo-LacNAcs and their sialylated analogs were synthesized.

Graphical abstract: Sequential one-pot enzymatic synthesis of oligo-N-acetyllactosamine and its multi-sialylated extensions

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Article information

DOI
https://doi.org/10.1039/C4CC01227E
Article type
Communication
Submitted
17 Feb 2014
Accepted
11 Apr 2014
First published
11 Apr 2014

Chem. Commun., 2014,50, 5786-5789

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Sequential one-pot enzymatic synthesis of oligo-N-acetyllactosamine and its multi-sialylated extensions

W. Chien, C. Liang, C. Yu, C. Lin, S. Li, I. Primadona, Y. Chen, K. K. T. Mong and C. Lin, Chem. Commun., 2014, 50, 5786 DOI: 10.1039/C4CC01227E

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