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Issue 43, 2014
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Biotin-conjugated N-methylisatoic anhydride: a chemical tool for nucleic acid separation by selective 2′-hydroxyl acylation of RNA

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Abstract

An isatoic anhydride derivative conjugated to a biotin and a disulfide linker was specifically designed for the separation of nucleic acids. Starting from a DNA–RNA mixture, a selective 2′-hydroxyl acylation of RNAs followed by capture with streptavidin-coated magnetic beads and cleavage of the disulfide led to elution of RNAs.

Graphical abstract: Biotin-conjugated N-methylisatoic anhydride: a chemical tool for nucleic acid separation by selective 2′-hydroxyl acylation of RNA

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Publication details

The article was received on 12 Feb 2014, accepted on 07 Apr 2014 and first published on 07 Apr 2014


Article type: Communication
DOI: 10.1039/C4CC01134A
Citation: Chem. Commun., 2014,50, 5748-5751
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    Biotin-conjugated N-methylisatoic anhydride: a chemical tool for nucleic acid separation by selective 2′-hydroxyl acylation of RNA

    S. Ursuegui, N. Chivot, S. Moutin, A. Burr, C. Fossey, T. Cailly, A. Laayoun, F. Fabis and A. Laurent, Chem. Commun., 2014, 50, 5748
    DOI: 10.1039/C4CC01134A

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