Issue 51, 2014
From the journal:

Chemical Communications

A rational approach for generating cardiac troponin I selective Spiegelmers†

Zsuzsanna Szeitner,a   Gergely Lautner,b   Szilvia K. Nagy,a   Róbert E. Gyurcsányi*b  and  Tamás Mészáros*ac  

* Corresponding authors

a Department of Medical Chemistry, Molecular Biology and Pathobiochemistry, Semmelweis University, Tűzoltó u. 37-47, H-1094 Budapest, Hungary
E-mail: meszaros.tamas@med.semmelweis-univ.hu

b MTA-BME Chemical Nanosensors Research Group, Department of Inorganic and Analytical Chemistry, Budapest University of Technology and Economics, Szent Gellért tér 4, H-1111 Budapest, Hungary
E-mail: robertgy@mail.bme.hu

c Technical Analytical Research Group of HAS, Szent Gellért tér 4, H-1111 Budapest, Hungary

Abstract

We report the first protein selective Spiegelmers of diagnostic relevance by rational identification of a target epitope and reverse screening of Spiegelmer candidates following the selection procedure. Application of the presented approach resulted in isolation of cardiac troponin I selective Spiegelmers with low nanomolar dissociation constant and functionality in serum.

Graphical abstract: A rational approach for generating cardiac troponin I selective Spiegelmers

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Article information

DOI
https://doi.org/10.1039/C4CC00447G
Article type
Communication
Submitted
18 Jan 2014
Accepted
06 May 2014
First published
06 May 2014
This article is Open Access
Creative Commons BY-NC license

Chem. Commun., 2014,50, 6801-6804

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A rational approach for generating cardiac troponin I selective Spiegelmers

Z. Szeitner, G. Lautner, S. K. Nagy, R. E. Gyurcsányi and T. Mészáros, Chem. Commun., 2014, 50, 6801 DOI: 10.1039/C4CC00447G

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