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Issue 38, 2014
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A rapid, site-selective and efficient route to the dual modification of DARPins

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Abstract

Designed ankyrin repeat proteins (DARPins) are valuable tools in both biochemistry and medicine. Herein we describe a rapid, simple method for the dual modification of DARPins by introduction of cysteine mutations at specific positions that results in a vast difference in their thiol nucleophilicity, allowing for clean sequential modification.

Graphical abstract: A rapid, site-selective and efficient route to the dual modification of DARPins

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Publication details

The article was received on 03 Jan 2014, accepted on 27 Mar 2014 and first published on 27 Mar 2014


Article type: Communication
DOI: 10.1039/C4CC00053F
Citation: Chem. Commun., 2014,50, 4898-4900
  • Open access: Creative Commons BY license
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    A rapid, site-selective and efficient route to the dual modification of DARPins

    P. Moody, V. Chudasama, R. I. Nathani, A. Maruani, S. Martin, M. E. B. Smith and S. Caddick, Chem. Commun., 2014, 50, 4898
    DOI: 10.1039/C4CC00053F

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