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Issue 19, 2014
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Streptavidin binding as a model to characterize thiol–ene chemistry-based polyamine surfaces for reversible photonic protein biosensing

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Abstract

Biotin- and iminobiotin-bonded surfaces obtained by thiol–ene chemistry and subsequent modification with polyamines were characterized with respect to streptavidin-binding capacity and reversibility for photonic biosensing using X-ray photoelectron spectroscopy and Mach–Zehnder-interferometric sensors. The streptavidin–iminobiotin system was exploited for reversible multilayer deposition and determination of affinity constants on each layer.

Graphical abstract: Streptavidin binding as a model to characterize thiol–ene chemistry-based polyamine surfaces for reversible photonic protein biosensing

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Publication details

The article was received on 12 Nov 2013, accepted on 25 Dec 2013 and first published on 09 Jan 2014


Article type: Communication
DOI: 10.1039/C3CC48640K
Citation: Chem. Commun., 2014,50, 2424-2427
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    Streptavidin binding as a model to characterize thiol–ene chemistry-based polyamine surfaces for reversible photonic protein biosensing

    E. Melnik, P. Muellner, O. Bethge, E. Bertagnolli, R. Hainberger and M. Laemmerhofer, Chem. Commun., 2014, 50, 2424
    DOI: 10.1039/C3CC48640K

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