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Issue 22, 2014
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Giant vesicles functionally expressing membrane receptors for an insect pheromone

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Abstract

To date, biochemical approaches to membrane receptors have been limited to the following methods: knockout or overexpression of membrane receptors by gene introduction and genome engineering or extraction of membrane receptor–surfactant complexes from innate cells and their introduction into model biomembranes. Here, we describe the development of a third method involving gene expression using cell-free in situ protein synthesis inside model biomembrane capsules. We verified this method by synthesizing olfactory receptors from the silkmoth Bombyx mori inside giant vesicles and found that they were excited in the presence of their ligand the Bombyx mori sex pheromone.

Graphical abstract: Giant vesicles functionally expressing membrane receptors for an insect pheromone

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Publication details

The article was received on 27 Oct 2013, accepted on 14 Jan 2014 and first published on 07 Feb 2014


Article type: Communication
DOI: 10.1039/C3CC48216B
Citation: Chem. Commun., 2014,50, 2958-2961
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    Giant vesicles functionally expressing membrane receptors for an insect pheromone

    S. Hamada, M. Tabuchi, T. Toyota, T. Sakurai, T. Hosoi, T. Nomoto, K. Nakatani, M. Fujinami and R. Kanzaki, Chem. Commun., 2014, 50, 2958
    DOI: 10.1039/C3CC48216B

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