Issue 27, 2014
From the journal:

Chemical Communications

A highly sensitive immunoassay using antibody-conjugated spherical mesoporous silica with immobilized enzymes†

Ji Young Eum,‡a   Sang Youn Hwang,‡a   Youngjun Ju,a   Jong Min Shim,b   Yunxian Piao,c   Jinwoo Lee,b   Hak-Sung Kimc  and  Jungbae Kim*a  

* Corresponding authors

a Department of Chemical and Biological Engineering, Korea University, Seoul 136-713, Republic of Korea
E-mail: jbkim3@korea.ac.kr

b Department of Chemical Engineering, Pohang University of Science and Technology, Pohang, Gyungbuk 790-784, Korea

c Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Daejeon 305-701, Korea

Abstract

A highly sensitive immunoassay was developed by using antibody-conjugated spherical mesoporous silica with immobilized enzymes. The higher ratio of enzyme/antibody than conventional ELISA improved both the sensitivity and dynamic range. Especially, the use of spherical mesoporous silica could achieve a limit of detection (LOD) with a sensitivity that is 20 times more than that of ELISA using amorphous silica.

Graphical abstract: A highly sensitive immunoassay using antibody-conjugated spherical mesoporous silica with immobilized enzymes

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Article information

DOI
https://doi.org/10.1039/C3CC48044E
Article type
Communication
Submitted
19 Oct 2013
Accepted
04 Dec 2013
First published
04 Dec 2013

Chem. Commun., 2014,50, 3546-3548

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A highly sensitive immunoassay using antibody-conjugated spherical mesoporous silica with immobilized enzymes

J. Y. Eum, S. Y. Hwang, Y. Ju, J. M. Shim, Y. Piao, J. Lee, H. Kim and J. Kim, Chem. Commun., 2014, 50, 3546 DOI: 10.1039/C3CC48044E

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