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Issue 40, 2014
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Label-free luminescence switch-on detection of T4 polynucleotide kinase activity using a G-quadruplex-selective probe

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Abstract

A label-free, oligonucleotide-based, switch-on luminescence detection method for T4 polynucleotide kinase activity has been developed using a novel G-quadruplex-selective luminescent Ir(III) complex probe. The application of the assay for screening potential T4 PNK inhibitors is also demonstrated. To our knowledge, this is the first metal-based assay for PNK activity.

Graphical abstract: Label-free luminescence switch-on detection of T4 polynucleotide kinase activity using a G-quadruplex-selective probe

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Publication details

The article was received on 29 Sep 2013, accepted on 25 Nov 2013 and first published on 26 Nov 2013


Article type: Communication
DOI: 10.1039/C3CC47444E
Citation: Chem. Commun., 2014,50, 5313-5315
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    Label-free luminescence switch-on detection of T4 polynucleotide kinase activity using a G-quadruplex-selective probe

    H. He, K. Leung, W. Wang, D. S. Chan, C. Leung and D. Ma, Chem. Commun., 2014, 50, 5313
    DOI: 10.1039/C3CC47444E

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