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Issue 2, 2014
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Activity-based diubiquitin probes for elucidating the linkage specificity of deubiquitinating enzymes

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Abstract

We report a new class of deubiquitinating enzyme (DUB) probes that resemble the native diubiquitin with a same linkage size and contain a Michael addition acceptor for trapping the DUB active-site cysteine. Both K63- and K48-linked diubiquitin probes were generated using a facile chemical ligation method. The diUb probes were demonstrated to label DUBs from different families and revealed intrinsic linkage specificities of DUBs.

Graphical abstract: Activity-based diubiquitin probes for elucidating the linkage specificity of deubiquitinating enzymes

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Publication details

The article was received on 26 Sep 2013, accepted on 22 Oct 2013 and first published on 13 Nov 2013


Article type: Communication
DOI: 10.1039/C3CC47382A
Citation: Chem. Commun., 2014,50, 216-218
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    Activity-based diubiquitin probes for elucidating the linkage specificity of deubiquitinating enzymes

    G. Li, Q. Liang, P. Gong, A. H. Tencer and Z. Zhuang, Chem. Commun., 2014, 50, 216
    DOI: 10.1039/C3CC47382A

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