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Issue 9, 2014
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Photo-regulation of constitutive gene expression in living cells by using ultrafast photo-cross-linking oligonucleotides

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Abstract

3-Cyanovinylcarbazole nucleoside (CNVK), which has an ultrafast photo-cross-linking ability to complementary RNA, was incorporated into the antisense oligonucleotide targeting green fluorescent protein (GFP) mRNA. We successfully demonstrated that the photoreactive antisense oligonucleotides effectively regulate GFP expression in a stable cell line expressing the GFP gene in a photo-responsive manner.

Graphical abstract: Photo-regulation of constitutive gene expression in living cells by using ultrafast photo-cross-linking oligonucleotides

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Publication details

The article was received on 14 Apr 2014, accepted on 13 Jun 2014 and first published on 26 Jun 2014


Article type: Communication
DOI: 10.1039/C4BM00117F
Author version available: Download Author version (PDF)
Citation: Biomater. Sci., 2014,2, 1154-1157
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    Photo-regulation of constitutive gene expression in living cells by using ultrafast photo-cross-linking oligonucleotides

    T. Sakamoto, A. Shigeno, Y. Ohtaki and K. Fujimoto, Biomater. Sci., 2014, 2, 1154
    DOI: 10.1039/C4BM00117F

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