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Issue 3, 2014
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A collagen-based corneal stroma substitute with micro-designed architecture

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Abstract

A 3D corneal stroma substitute with micro-level patterns was constructed from a stack of 4 micro patterned collagen or collagen–elastin like recombinamer (Col:ELR) blend layers. The transparency of all of the films was quite high with the uncrosslinked (UXL) films and dehydrothermally treated (150 °C, 24 h) Col:ELR films yielding the best results. Human corneal keratocytes (HK) could be attached and proliferated equally well on the single films of Col and Col:ELR. However, for the multilayer constructs the proliferation was higher on Col than on Col:ELR. The cells were found to align along the patterns (microchannels with a 39 μm groove depth, 8 μm groove width, 3.3 μm ridge width, and 54.7° inclination angle) of the films, while no significant alignment was observed on the unpatterned films. The transparency of the seeded Col:ELR films was superior to the Col films over a 30 day incubation period and was quite close to that of a native human cornea. It was concluded that the Col and Col:ELR films and their 3D constructs have significant potential for use as corneal stroma substitutes.

Graphical abstract: A collagen-based corneal stroma substitute with micro-designed architecture

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Publication details

The article was received on 16 Aug 2013, accepted on 30 Sep 2013 and first published on 01 Nov 2013


Article type: Paper
DOI: 10.1039/C3BM60194C
Citation: Biomater. Sci., 2014,2, 318-329
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    A collagen-based corneal stroma substitute with micro-designed architecture

    C. Kilic, A. Girotti, J. C. Rodriguez-Cabello and V. Hasirci, Biomater. Sci., 2014, 2, 318
    DOI: 10.1039/C3BM60194C

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