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Issue 16, 2014
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Automated enzyme-linked immunosorbent assay using beads in a single tip (BIST) technology coupled with a novel anchor protein for oriented antibody immobilization

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Abstract

An automated enzyme-linked immunosorbent assay (ELISA) system was developed using beads in a single tip (BIST) technology. Sandwich ELISA was performed on glass beads functionalized with a capture antibody via the fusion of antibody-binding streptococcal protein G and a silica/glass-binding protein Si-tag. Our system enables rapid, sensitive detection of antigens.

Graphical abstract: Automated enzyme-linked immunosorbent assay using beads in a single tip (BIST) technology coupled with a novel anchor protein for oriented antibody immobilization

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Publication details

The article was received on 11 Mar 2014, accepted on 29 Apr 2014 and first published on 30 Apr 2014


Article type: Communication
DOI: 10.1039/C4AY00614C
Citation: Anal. Methods, 2014,6, 6232-6235
  • Open access: Creative Commons BY license
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    Automated enzyme-linked immunosorbent assay using beads in a single tip (BIST) technology coupled with a novel anchor protein for oriented antibody immobilization

    T. Ikeda, T. Ueda, H. Tajima, K. Sekiguchi and A. Kuroda, Anal. Methods, 2014, 6, 6232
    DOI: 10.1039/C4AY00614C

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