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Issue 2, 2014
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On the applicability of SiO2/Al2O3/Nb2O5 and SiO2/Al2O3/TiO2 as a biocompatible platform for chloroperoxidase

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Abstract

In the present work, two mixed oxides, namely, SiO2/Al2O3/Nb2O5 and SiO2/Al2O3/TiO2 (designated as SiAlNb and SiAlTi, respectively), obtained using the sol–gel method were used to immobilize chloroperoxidase. Hydrogen peroxide was quantified using potassium hexacyanoferrate(II) as a redox-mediator and amperometric measurements at 0.0 V vs. Ag/AgCl/Cl (3 M). The SiAlTi biosensor presented higher sensitivity than the SiAlNb biosensor, however, the first one did not present a good response regarding time. The developed biosensor using the SiAlNb mixed oxide provided good signal levels, good linearity, good stability (retaining approximately 70% of its original response after 6 weeks of usage), a low detection limit (3 μM), good sensitivity, a suitable working range (from 4 to 19 μM), fast response and good repeatability. The recovery of the amperometric method for the detection of hydrogen peroxide in synthetic samples was approximately 100 ± 2%, and for Listerine® Whitening Pre-Brush Rinse samples fortified with 1, 2 and 3% (v/v) of hydrogen peroxide, it was 100 ± 3%.

Graphical abstract: On the applicability of SiO2/Al2O3/Nb2O5 and SiO2/Al2O3/TiO2 as a biocompatible platform for chloroperoxidase

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Publication details

The article was received on 26 Aug 2013, accepted on 28 Oct 2013 and first published on 29 Oct 2013


Article type: Paper
DOI: 10.1039/C3AY41468J
Citation: Anal. Methods, 2014,6, 521-528
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    On the applicability of SiO2/Al2O3/Nb2O5 and SiO2/Al2O3/TiO2 as a biocompatible platform for chloroperoxidase

    M. S. Ribeiro, F. Jorge de Vasconcellos Júnior, B. Teixeira da Fonseca, F. Carvalho de Souza, F. D. Rojas Soares, É. C. Lima, M. F. Cabral, E. S. Ribeiro and E. D'Elia, Anal. Methods, 2014, 6, 521
    DOI: 10.1039/C3AY41468J

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