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Issue 10, 2014
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Recent advances in sample preparation techniques to overcome difficulties encountered during quantitative analysis of small molecules from biofluids using LC-MS/MS

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Abstract

Liquid chromatography-mass spectrometry analysis of small molecules from biofluids requires sensitive and robust assays. Because of the very complex nature of many biological samples, efficient sample preparation protocols to remove unwanted components and to selectively extract the compounds of interest are an essential part of almost every bioanalytical workflow. This review describes the most common problems encountered during sample preparation, ways to optimize established sample preparation techniques and important recent developments to reduce or eliminate major interferents from biofluids.

Graphical abstract: Recent advances in sample preparation techniques to overcome difficulties encountered during quantitative analysis of small molecules from biofluids using LC-MS/MS

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Publication details

The article was received on 14 Jan 2014, accepted on 12 Feb 2014 and first published on 13 Feb 2014


Article type: Minireview
DOI: 10.1039/C4AN00094C
Citation: Analyst, 2014,139, 2265-2276
  • Open access: Creative Commons BY license
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    Recent advances in sample preparation techniques to overcome difficulties encountered during quantitative analysis of small molecules from biofluids using LC-MS/MS

    C. Bylda, R. Thiele, U. Kobold and D. A. Volmer, Analyst, 2014, 139, 2265
    DOI: 10.1039/C4AN00094C

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