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Issue 22, 2014
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Molecular phenotypic profiling of a Saccharomyces cerevisiae strain at the single-cell level

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Abstract

Studying cell-to-cell heterogeneity requires techniques which robustly deliver reproducible results with single-cell sensitivity. Through a new fabrication method for the microarrays for mass spectrometry (MAMS) platform, we now have attained robustness and reproducibility in our single-cell level mass spectrometry measurements that allowed us to combine single-cell MAMS-based measurements from different days and samples. By combining multiple measurements, we were able to identify three co-existing phenotypes in an isogenic population of Saccharomyces cerevisiae characterized by distinctively different levels of glycolytic intermediates.

Graphical abstract: Molecular phenotypic profiling of a Saccharomyces cerevisiae strain at the single-cell level

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Publication details

The article was received on 22 Jun 2014, accepted on 21 Aug 2014 and first published on 22 Aug 2014


Article type: Paper
DOI: 10.1039/C4AN01119H
Citation: Analyst, 2014,139, 5709-5717
  • Open access: Creative Commons BY license
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    Molecular phenotypic profiling of a Saccharomyces cerevisiae strain at the single-cell level

    A. M. Schmidt, S. R. Fagerer, K. Jefimovs, F. Buettner, C. Marro, E. C. Siringil, K. L. Boehlen, M. Pabst and A. J. Ibáñez, Analyst, 2014, 139, 5709
    DOI: 10.1039/C4AN01119H

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