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Issue 19, 2014
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Diagnosing malaria infected cells at the single cell level using focal plane array Fourier transform infrared imaging spectroscopy

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Abstract

New methods are needed to rapidly identify malaria parasites in blood smears. The coupling of a Focal Plane Array (FPA) infrared microscope system to a synchrotron light source at IRENI enables rapid molecular imaging at high spatial resolution. The technique, in combination with hyper-spectral processing, enables imaging and diagnosis of early stage malaria parasites at the single cell level in a blood smear. The method relies on the detection of distinct lipid signatures associated with the different stages of the malaria parasite and utilises resonant Mie extended multiplicative scatter correction to pre-process the spectra followed by full bandwidth image deconvolution to resolve the single cells. This work demonstrates the potential of focal plane technology to diagnose single cells in a blood smear. Brighter laboratory based infrared sources, optical refinements and higher sensitive detectors will soon see the emergence of focal plane array imaging in the clinical environment.

Graphical abstract: Diagnosing malaria infected cells at the single cell level using focal plane array Fourier transform infrared imaging spectroscopy

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Publication details

The article was received on 30 May 2014, accepted on 14 Jul 2014 and first published on 14 Jul 2014


Article type: Communication
DOI: 10.1039/C4AN00989D
Citation: Analyst, 2014,139, 4769-4774
  • Open access: Creative Commons BY license
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    Diagnosing malaria infected cells at the single cell level using focal plane array Fourier transform infrared imaging spectroscopy

    B. R. Wood, Keith. R. Bambery, M. W. A. Dixon, L. Tilley, M. J. Nasse, E. Mattson and C. J. Hirschmugl, Analyst, 2014, 139, 4769
    DOI: 10.1039/C4AN00989D

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