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Issue 19, 2014
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A spatiotemporally defined in vitro microenvironment for controllable signal delivery and drug screening

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Abstract

Cancer metastasis and drug resistance are important malignant tumor phenotypes that cause roughly 90% mortality in human cancers. Current therapeutic strategies, however, face substantial challenges partially due to a lack of applicable pre-clinical models and drug-screening platforms. Notably, microscale and three-dimensional (3D) tissue culture platforms capable of mimicking in vivo microenvironments to replicate physiological conditions have become vital tools in a wide range of cellular and clinical studies. Here, we present a microfluidic device capable of mimicking a configurable tumor microenvironment to study in vivo-like cancer cell migration as well as screening of inhibitors on both parental tumors and migratory cells. In addition, a novel evaporation-based paper pump was demonstrated to achieve adaptable and sustainable concentration gradients for up to 6 days in this model. This straightforward modeling approach allows for fast patterning of a wide variety of cell types in 3D and may be further integrated into biological assays. We also demonstrated cell migration from tumor spheroids induced by an epidermal growth factor (EGF) gradient and exhibited lowered expression of an epithelial marker (EpCAM) compared with parental cells, indicative of partial epithelial-mesenchymal transition (EMT) in this process. Importantly, pseudopodia protrusions from the migratory cells – critical during cancer metastasis – were demonstrated. Insights gained from this work offer new opportunities to achieve active control of in vitro tumor microenvironments on-demand, and may be amenable towards tailored clinical applications.

Graphical abstract: A spatiotemporally defined in vitro microenvironment for controllable signal delivery and drug screening

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Publication details

The article was received on 23 May 2014, accepted on 02 Jul 2014 and first published on 03 Jul 2014


Article type: Paper
DOI: 10.1039/C4AN00936C
Author version available: Download Author version (PDF)
Citation: Analyst, 2014,139, 4846-4854
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    A spatiotemporally defined in vitro microenvironment for controllable signal delivery and drug screening

    C. Kuo, H. Liu, G. Huang, C. Chang, C. Chen, K. Chen, R. Yun-Ju Huang, C. Lin, H. Lee, C. Huang and A. M. Wo, Analyst, 2014, 139, 4846
    DOI: 10.1039/C4AN00936C

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