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Issue 18, 2014
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On-chip monitoring of skeletal myoblast transplantation for the treatment of hypoxia-induced myocardial injury

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Abstract

A comprehensive elucidation of the unexpected adverse events that occur in skeletal myoblast transplantation is fundamental for the optimization of myocardial therapeutic effects. However, a well-defined method to study the interactions between skeletal myoblasts and cardiomyocytes during the healing process is out of reach. Here, we describe a microfluidic method for monitoring the interactions between skeletal myoblasts and hypoxia-injured cardiomyocytes in a spatiotemporally-controlled manner, mimicking the in vivo cell transplantation process. A myocardial hypoxia environment was created using an oxygen consumption blocking reagent, carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone. Meanwhile, the interactions between the skeletal L6 myoblasts and hypoxia-injured myocardium H9c2 cells were investigated, and the effects of a L6 conditional medium on H9c2 cells were comparatively analyzed by quantitatively measuring the morphological and pathophysiological dynamics of H9c2 cells. The results showed that skeletal myoblasts could repair hypoxia-injured H9c2 cells mainly through direct cell-to-cell interactions. This simple on-chip assay for investigating myocardial repair processes may provide avenues for the in vitro screening of drug-induced cardiotoxicity.

Graphical abstract: On-chip monitoring of skeletal myoblast transplantation for the treatment of hypoxia-induced myocardial injury

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Publication details

The article was received on 18 Apr 2014, accepted on 19 Jun 2014 and first published on 20 Jun 2014


Article type: Paper
DOI: 10.1039/C4AN00697F
Author version available: Download Author version (PDF)
Citation: Analyst, 2014,139, 4482-4490
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    On-chip monitoring of skeletal myoblast transplantation for the treatment of hypoxia-induced myocardial injury

    J. He, C. Ma, W. Liu and J. Wang, Analyst, 2014, 139, 4482
    DOI: 10.1039/C4AN00697F

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