Jump to main content
Jump to site search

Issue 13, 2014
Previous Article Next Article

Micropatterning neuronal networks

Author affiliations

Abstract

Spatially organised neuronal networks have wide reaching applications, including fundamental research, toxicology testing, pharmaceutical screening and the realisation of neuronal implant interfaces. Despite the large number of methods catalogued in the literature there remains the need to identify a method that delivers high pattern compliance, long-term stability and is widely accessible to neuroscientists. In this comparative study, aminated (polylysine/polyornithine and aminosilanes) and cytophobic (poly(ethylene glycol) (PEG) and methylated) material contrasts were evaluated. Backfilling plasma stencilled PEGylated substrates with polylysine does not produce good material contrasts, whereas polylysine patterned on methylated substrates becomes mobilised by agents in the cell culture media which results in rapid pattern decay. Aminosilanes, polylysine substitutes, are prone to hydrolysis and the chemistries prove challenging to master. Instead, the stable coupling between polylysine and PLL-g-PEG can be exploited: Microcontact printing polylysine onto a PLL-g-PEG coated glass substrate provides a simple means to produce microstructured networks of primary neurons that have superior pattern compliance during long term (>1 month) culture.

Graphical abstract: Micropatterning neuronal networks

Back to tab navigation

Supplementary files

Publication details

The article was received on 04 Apr 2014, accepted on 19 May 2014 and first published on 19 May 2014


Article type: Paper
DOI: 10.1039/C4AN00608A
Author version available: Download Author version (PDF)
Citation: Analyst, 2014,139, 3256-3264
  •   Request permissions

    Micropatterning neuronal networks

    H. Hardelauf, S. Waide, J. Sisnaiske, P. Jacob, V. Hausherr, N. Schöbel, D. Janasek, C. van Thriel and J. West, Analyst, 2014, 139, 3256
    DOI: 10.1039/C4AN00608A

Search articles by author

Spotlight

Advertisements