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Issue 5, 2014
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Human plasma stability during handling and storage: impact on NMR metabolomics

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Abstract

This work contributes to fill in some existing gaps in the knowledge of human plasma degradability during handling and storage, a paramount issue in Nuclear Magnetic Resonance (NMR) metabolomics. Regarding the comparison between heparin and EDTA anti-coagulant collection tubes, the former showed no interference of the polysaccharide, while conserving full spectral information. In relation to time/temperature conditions, room temperature was seen to have a large impact on lipoproteins and choline compounds from 2.5 hours. In addition, short-term storage at −20 °C was found suitable up to 7 days but, for periods up to 1 month, −80 °C was recommended. Furthermore, in the case of reusing plasma samples, no more than 3 consecutive freeze–thaw cycles were found advisable. Finally, the impact of long-term −80 °C storage (up to 2.5 years) was found almost negligible, as evaluated on a partially matched non-fasting cohort (n = 49), after having investigated the possible confounding nature of the particular non-fasting conditions employed.

Graphical abstract: Human plasma stability during handling and storage: impact on NMR metabolomics

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Publication details

The article was received on 25 Nov 2013, accepted on 06 Jan 2014 and first published on 07 Jan 2014


Article type: Paper
DOI: 10.1039/C3AN02188B
Author version available: Download Author version (PDF)
Citation: Analyst, 2014,139, 1168-1177
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    Human plasma stability during handling and storage: impact on NMR metabolomics

    J. Pinto, M. R. M. Domingues, E. Galhano, C. Pita, M. D. C. Almeida, I. M. Carreira and A. M. Gil, Analyst, 2014, 139, 1168
    DOI: 10.1039/C3AN02188B

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