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Issue 9, 2014
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In-solution multiplex miRNA detection using DNA-templated silver nanocluster probes

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Abstract

MicroRNAs (miRNAs) are small regulatory RNAs (size ∼21nt to ∼25nt) that can be used as biomarkers of disease diagnosis, and efforts have been directed towards the invention of a rapid, simple and sequence-selective detection method for miRNAs. We recently developed a DNA/silver nanoclusters (AgNCs)-based turn-off fluorescence method in the presence of target miRNA. To further advance our method toward multiplex miRNA detection in solution, the design of various fluorescent DNA/AgNCs probes was essential. Therefore, tethering of DNA-12nt scaffolds with 9 different AgNCs emitters to target-sensing DNA sequences was investigated. Interestingly, for the creation of spectrally different DNA/AgNCs probes, not only were the emitters encapsulated in 9 different DNA-12nt scaffolds necessary but the tethered target-sensing DNA sequences are also crucial to tune the fluorescence across the visible to infra-red region. In this study, we obtained three spectrally distinctive emitters of each DNA/AgNCs probes such as green, red, and near-infrared (NIR) fluorescence. Using these DNA/AgNCs probes, we here show a proof of concept for a rapid, one-step, in-solution multiplex miRNA detection method.

Graphical abstract: In-solution multiplex miRNA detection using DNA-templated silver nanocluster probes

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Publication details

The article was received on 20 Nov 2013, accepted on 23 Jan 2014 and first published on 24 Jan 2014


Article type: Paper
DOI: 10.1039/C3AN02150E
Author version available: Download Author version (PDF)
Citation: Analyst, 2014,139, 2158-2166
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    In-solution multiplex miRNA detection using DNA-templated silver nanocluster probes

    P. Shah, P. W. Thulstrup, S. K. Cho, Y. Bhang, J. C. Ahn, S. W. Choi, M. J. Bjerrum and S. W. Yang, Analyst, 2014, 139, 2158
    DOI: 10.1039/C3AN02150E

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