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Issue 46, 2013
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Visualizing the localization of transfection complexes during graphene nanoparticle-based transfection

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Abstract

Recent research has shown that ultra-small graphene oxide (USGO) particles functionalized with polyethylenimine (PEI) can be used to transfect human cell lines with very high efficiency and low toxicity. In this study, confocal fluorescence microscopy is used to provide more insight into the nature of the complexes formed by DNA and USGO_PEI and to track their localization during the transfection process. The results indicate that the DNA enters the nucleus in complex with the USGO_PEI, which remains inside the nucleus. Also, it is observed that USGO_PEI and plasmid DNA form large aggregates in the transfection medium. Both observations raise concerns that this type of nanomaterial might need further improvements for therapeutic use.

Graphical abstract: Visualizing the localization of transfection complexes during graphene nanoparticle-based transfection

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Publication details

The article was received on 29 Sep 2013, accepted on 14 Oct 2013 and first published on 16 Oct 2013


Article type: Communication
DOI: 10.1039/C3TB21349H
Citation: J. Mater. Chem. B, 2013,1, 6353-6358
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    Visualizing the localization of transfection complexes during graphene nanoparticle-based transfection

    F. T. Bruggencate, F. Laroche, Y. Zhang, G. Song, S. Yin, J. P. Abrahams and Z. Liu, J. Mater. Chem. B, 2013, 1, 6353
    DOI: 10.1039/C3TB21349H

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