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Issue 30, 2013
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Development of LbL biopolymer capsules as a delivery system for the multilayer-assembled anti-inflammatory substance α1-antitrypsin

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Abstract

The treatment of chronic inflammation requires new concepts since recent approaches are mostly accompanied by massive side effects. Layer-by-layer (LbL) microcapsules, functionalized with anti-inflammatory substances such as α1-antitrypsin (AT), may avoid major side- and off-target effects thanks to their local application and the sustained delivery of defined amounts of the active agents into polymorphonuclear leukocytes (PMNs). However, LbL microcapsule application in inflamed tissues requires specific design and preparation. High biocompatibility has to be guaranteed by using biopolymers and ensuring the complete dissolution of the particle core. Moreover, off-target effects – such as macrophage-mediated pro-inflammatory signaling – have to be avoided. In our approach, biopolymer-coated CaCO3 particles were used and the core dissolution process was optimized to obtain highly biocompatible, non-aggregated, long-time stable and clearly calcium-free capsules. A fast verification tool was applied to monitor the remaining Ca2+ content. The incubation with macrophages shows reduced pro-inflammatory signaling compared to microparticles. Regarding their performance as a drug delivery system, AT-functionalized capsules showed a high inhibiting capacity towards neutrophil elastase, a major degradative enzyme in chronic inflammation. Consequently, the optimized design and preparation methods described in this study provide the basis for the development of medically applicable LbL carrier systems for active agents in the treatment of inflammatory processes.

Graphical abstract: Development of LbL biopolymer capsules as a delivery system for the multilayer-assembled anti-inflammatory substance α1-antitrypsin

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Publication details

The article was received on 20 Mar 2013, accepted on 29 May 2013 and first published on 30 May 2013


Article type: Paper
DOI: 10.1039/C3TB20390E
Citation: J. Mater. Chem. B, 2013,1, 3633-3643
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    Development of LbL biopolymer capsules as a delivery system for the multilayer-assembled anti-inflammatory substance α1-antitrypsin

    V. Strehlow, J. Lessig, M. Göse and U. Reibetanz, J. Mater. Chem. B, 2013, 1, 3633
    DOI: 10.1039/C3TB20390E

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