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Issue 15, 2013
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A gold nanoparticle-enhanced fluorescence polarization biosensor for amplified detection of T4 polynucleotide kinase activity and inhibition

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Abstract

A novel fluorescence polarization (FP) nanosensor based on λ exonuclease cleavage reaction and FP enhancement effect of gold nanoparticles (AuNPs) has been developed for the detection of T4 polynucleotide kinase activity and inhibition. This FP sensor exhibits higher detection sensitivity over traditional fluorescence sensors by two orders of magnitude.

Graphical abstract: A gold nanoparticle-enhanced fluorescence polarization biosensor for amplified detection of T4 polynucleotide kinase activity and inhibition

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Publication details

The article was received on 07 Jan 2013, accepted on 04 Mar 2013 and first published on 05 Mar 2013


Article type: Communication
DOI: 10.1039/C3TB00025G
Citation: J. Mater. Chem. B, 2013,1, 2018-2021
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    A gold nanoparticle-enhanced fluorescence polarization biosensor for amplified detection of T4 polynucleotide kinase activity and inhibition

    Y. Huang, J. Chen, M. Shi, S. Zhao, Z. Chen and H. Liang, J. Mater. Chem. B, 2013, 1, 2018
    DOI: 10.1039/C3TB00025G

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