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Issue 47, 2013
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Achievement of cornea-like organizations in dense collagen I solutions: clues to the physico-chemistry of cornea morphogenesis

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Abstract

Multiphoton and electron microscopic analyses show that acido-soluble collagen I prepared in 5 mM acetic acid (pH 3.5) at concentration above 45 mg mL−1 spontaneously generates liquid crystal phases mimicking plywood organization found in cornea tissues. Those organizations extend for several hundred micrometers. Transmission electron microscopy reveals the presence of small nanofibrils organized in a complex phase, coupling overall smectic and cholesteric organizations together with local order. Those nanofibrils could be the mesogen elements giving rise to this plywood organization. These data provide clues to physico-chemical events that may take place in cornea morphogenesis in vivo. This result is invaluable for bioengineering fields, as this liquid crystal organization paves the way for the generation of collagen based bio-mimetic cornea matrices.

Graphical abstract: Achievement of cornea-like organizations in dense collagen I solutions: clues to the physico-chemistry of cornea morphogenesis

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Publication details

The article was received on 02 Aug 2013, accepted on 09 Oct 2013 and first published on 10 Oct 2013


Article type: Paper
DOI: 10.1039/C3SM52097H
Citation: Soft Matter, 2013,9, 11241-11248
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    Achievement of cornea-like organizations in dense collagen I solutions: clues to the physico-chemistry of cornea morphogenesis

    P. De Sa Peixoto, A. Deniset-Besseau, M. Schmutz, A. Anglo, C. Illoul, M. Schanne-Klein and G. Mosser, Soft Matter, 2013, 9, 11241
    DOI: 10.1039/C3SM52097H

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