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Issue 45, 2013
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Monolayer spontaneous curvature of raft-forming membrane lipids

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Abstract

Monolayer spontaneous curvatures for cholesterol, DOPE, POPE, DOPC, DPPC, DSPC, POPC, SOPC, and egg sphingomyelin were obtained using small-angle X-ray scattering (SAXS) on inverted hexagonal phases (HII). Spontaneous curvatures of bilayer forming lipids were estimated by adding controlled amounts to a HII forming template following previously established protocols. Spontaneous curvatures of both phosphatidylethanolamines and cholesterol were found to be at least a factor of two more negative than those of phosphatidylcholines, whose J0 values are closer to zero. Interestingly, a significant positive J0 value was retrieved for DPPC. We further determined the temperature dependence of the spontaneous curvatures J0(T) in the range from 15 to 55 °C, resulting in a quite narrow distribution of −1 to −3 × 10−3 (nm °C)−1 for most investigated lipids. The data allowed us to estimate the monolayer spontaneous curvatures of ternary lipid mixtures showing liquid ordered/liquid disordered phase coexistence. We report spontaneous curvature phase diagrams for DSPC/DOPC/Chol, DPPC/DOPC/Chol and SM/POPC/Chol and discuss effects on protein insertion and line tension.

Graphical abstract: Monolayer spontaneous curvature of raft-forming membrane lipids

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Publication details

The article was received on 04 Jul 2013, accepted on 24 Sep 2013 and first published on 27 Sep 2013


Article type: Paper
DOI: 10.1039/C3SM51829A
Citation: Soft Matter, 2013,9, 10877-10884
  • Open access: Creative Commons BY-NC license
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    Monolayer spontaneous curvature of raft-forming membrane lipids

    B. Kollmitzer, P. Heftberger, M. Rappolt and G. Pabst, Soft Matter, 2013, 9, 10877
    DOI: 10.1039/C3SM51829A

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