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Issue 45, 2013
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Motility fractionation of bacteria by centrifugation

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Abstract

Centrifugation is a widespread laboratory technique used to separate mixtures into fractions characterized by a specific size, weight or density. We demonstrate that centrifugation can also be used to separate swimming cells having different motilities. To do this we study self-propelled bacteria under the influence of an external centrifugal field. Using dynamic image correlation spectroscopy we measure the spatially resolved motility of bacteria after centrifugation. A significant gradient in swimming-speeds is observed for increasing centrifugal speeds. Our results can be reproduced by a model that treats bacteria as “hot” colloidal particles having a diffusion coefficient that depends on the swimming speed.

Graphical abstract: Motility fractionation of bacteria by centrifugation

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Publication details

The article was received on 02 May 2013, accepted on 27 Sep 2013 and first published on 27 Sep 2013


Article type: Paper
DOI: 10.1039/C3SM51223A
Citation: Soft Matter, 2013,9, 10885-10890
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    Motility fractionation of bacteria by centrifugation

    C. Maggi, A. Lepore, J. Solari, A. Rizzo and R. Di Leonardo, Soft Matter, 2013, 9, 10885
    DOI: 10.1039/C3SM51223A

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