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Issue 10, 2013
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Repair of large area pores in supported double bilayers

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Abstract

We describe an experimental system where we can generate, and subsequently close, multiple large membrane ruptures in supported double bilayers. We show in this study for the first time that large membrane pores (∼10–150 μm in size) in flat phospholipid vesicles can be reduced in size or completely closed by a pore edge tension driven area reduction mechanism. We can dynamically control the membrane tension of a flat giant unilamellar vesicle and its interplay with the surface adhesion to a solid support. Adhesion to the support surface causes increased membrane tension, which eventually relaxes by the formation of several pores in the membrane. We show that the tension propagation time τmax is exceptionally long in this system, which allows for simultaneous opening of multiple pores. The pores can be stabilized by Ca2+-mediated pinning sites in the interior of the flat giant unilamellar vesicle. After pore formation followed by pinning, we depleted Ca2+ ions resulting in removal of pinning and relaxation of membrane tension. This allows the pore to close, driven by the pore edge tension.

Graphical abstract: Repair of large area pores in supported double bilayers

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Publication details

The article was received on 21 Oct 2012, accepted on 07 Jan 2013 and first published on 28 Jan 2013


Article type: Communication
DOI: 10.1039/C3SM27429B
Citation: Soft Matter, 2013,9, 2787-2792
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    Repair of large area pores in supported double bilayers

    I. Gözen, B. Ortmen, I. Põldsalu, P. Dommersnes, O. Orwar and A. Jesorka, Soft Matter, 2013, 9, 2787
    DOI: 10.1039/C3SM27429B

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