Jump to main content
Jump to site search

Issue 10, 2013
Previous Article Next Article

Self-assembled light-driven photosynthetic-respiratory electron transport chain hybrid proton pump

Author affiliations

Abstract

One of the fundamental biochemical reactions providing the foundation of life is photosynthesis occurring in plants and cyanobacteria. Photon energy is converted into stored chemical energy which flows through various metabolic processes that in the case of most aerobic organisms, terminates in the electron transport chain. In this study we demonstrate a synthetic hybrid photosynthetic-respiratory chain based on ruthenium(II)-terpyridine linked to cytochrome c and coupled with cytochrome c oxidase. Upon irradiation at an initial pH of 7.2, we observe proton pumping at a rate of ≈3.3 × 103 H+ per s across the polymer bilayer generating a gradient up to ΔpH ≈ 0.2. Remarkably, cytochrome c oxidase appears to have a greater than 50% native mitochondrial orientation in the polymersome membrane. Our photoactive hybrid electron-proton chain generates a proton gradient that can store chemical energy which is a step towards the development of a model artificial protocell.

Graphical abstract: Self-assembled light-driven photosynthetic-respiratory electron transport chain hybrid proton pump

Back to tab navigation

Supplementary files

Publication details

The article was received on 26 Jun 2013, accepted on 18 Jul 2013 and first published on 19 Jul 2013


Article type: Edge Article
DOI: 10.1039/C3SC51780B
Citation: Chem. Sci., 2013,4, 3833-3838
  •   Request permissions

    Self-assembled light-driven photosynthetic-respiratory electron transport chain hybrid proton pump

    D. Hvasanov, J. R. Peterson and P. Thordarson, Chem. Sci., 2013, 4, 3833
    DOI: 10.1039/C3SC51780B

Search articles by author

Spotlight

Advertisements