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Issue 9, 2013
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Glycomimetic affinity-enrichment proteomics identifies partners for a clinically-utilized iminosugar

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Abstract

Widescale evaluation of interacting partners for carbohydrates is an underexploited area. Probing of the ‘glyco-interactome’ has particular relevance given the lack of direct genetic control of glycoconjugate biosynthesis. Here we design, create and utilize a natural product-derived glycomimetic iminosugar probe in a Glycomimetic Affinity-enrichment Proteomics (Glyco-AeP) strategy to elucidate key interactions directly from mammalian tissue. The binding partners discovered here and the associated genomic analysis implicate a subset of chaperone and junctional proteins as important in male fertility. Such repurposing of existing therapeutics thus creates direct routes to probing in vivo function. The success of this strategy suggests a general approach to discovering ‘carbohydrate-active’ partners in biology.

Graphical abstract: Glycomimetic affinity-enrichment proteomics identifies partners for a clinically-utilized iminosugar

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Publication details

The article was received on 26 Mar 2013, accepted on 04 Jul 2013 and first published on 08 Jul 2013


Article type: Edge Article
DOI: 10.1039/C3SC50826A
Citation: Chem. Sci., 2013,4, 3442-3446
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    Glycomimetic affinity-enrichment proteomics identifies partners for a clinically-utilized iminosugar

    I. N. Cruz, C. S. Barry, H. B. Kramer, C. C. Chuang, S. Lloyd, A. C. van der Spoel, F. M. Platt, M. Yang and B. G. Davis, Chem. Sci., 2013, 4, 3442
    DOI: 10.1039/C3SC50826A

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