Jump to main content
Jump to site search

Issue 2, 2014
Previous Article Next Article

Cytochrome P450-catalyzed insertion of carbenoids into N–H bonds

Author affiliations

Abstract

Expanding nature's catalytic repertoire to include reactions important in synthetic chemistry will open new opportunities for ‘green’ chemistry and biosynthesis. We demonstrate the first enzyme-catalyzed insertion of carbenoids into N–H bonds. This type of bond disconnection, which has no counterpart in nature, can be mediated by variants of the cytochrome P450 from Bacillus megaterium. The N–H insertion reaction takes place in water, provides the desired products in 26–83% yield, forms the single addition product exclusively, and does not require slow addition of the diazo component.

Graphical abstract: Cytochrome P450-catalyzed insertion of carbenoids into N–H bonds

Back to tab navigation

Supplementary files

Publication details

The article was received on 09 Sep 2013, accepted on 01 Nov 2013 and first published on 04 Nov 2013


Article type: Edge Article
DOI: 10.1039/C3SC52535J
Citation: Chem. Sci., 2014,5, 598-601
  •   Request permissions

    Cytochrome P450-catalyzed insertion of carbenoids into N–H bonds

    Z. J. Wang, N. E. Peck, H. Renata and F. H. Arnold, Chem. Sci., 2014, 5, 598
    DOI: 10.1039/C3SC52535J

Search articles by author

Spotlight

Advertisements