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Issue 9, 2013
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A novel approach to the site-selective dual labelling of a protein via chemoselective cysteine modification

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Abstract

Local protein microenvironment is used to control the outcome of reaction between cysteine residues and 2,5-dibromohexanediamide. The differential reactivity is exploited to introduce two orthogonal reactive handles onto the surface of a double cysteine mutant of superfolder green fluorescent protein in a regioselective manner. Subsequent elaboration with commonly used thiol and alkyne containing reagents affects site-selective protein dual labelling.

Graphical abstract: A novel approach to the site-selective dual labelling of a protein via chemoselective cysteine modification

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Publication details

The article was received on 13 May 2013, accepted on 17 Jun 2013 and first published on 18 Jun 2013


Article type: Edge Article
DOI: 10.1039/C3SC51333E
Citation: Chem. Sci., 2013,4, 3455-3458
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    A novel approach to the site-selective dual labelling of a protein via chemoselective cysteine modification

    R. I. Nathani, P. Moody, V. Chudasama, M. E. B. Smith, R. J. Fitzmaurice and S. Caddick, Chem. Sci., 2013, 4, 3455
    DOI: 10.1039/C3SC51333E

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