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Issue 13, 2013
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Synthesis and enzymatic incorporation of norbornene-modified nucleoside triphosphates for Diels–Alder bioconjugation

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Abstract

Norbornene-modified nucleoside triphosphates were synthesised and enzymatically incorporated into DNA via primer extension and polymerase chain reaction. DNA strands containing several hundred norbornene moieties were prepared and successfully labelled utilising the inverse-electron-demand Diels–Alder reaction.

Graphical abstract: Synthesis and enzymatic incorporation of norbornene-modified nucleoside triphosphates for Diels–Alder bioconjugation

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Publication details

The article was received on 28 Sep 2012, accepted on 24 Jan 2013 and first published on 28 Jan 2013


Article type: Communication
DOI: 10.1039/C3RA40373D
Citation: RSC Adv., 2013,3, 4181-4183
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    Synthesis and enzymatic incorporation of norbornene-modified nucleoside triphosphates for Diels–Alder bioconjugation

    J. Schoch and A. Jäschke, RSC Adv., 2013, 3, 4181
    DOI: 10.1039/C3RA40373D

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