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Issue 15, 2013
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Preparation of liposomes: a novel application of microengineered membranes - investigation of the process parameters and application to the encapsulation of vitamin E

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Abstract

Liposomes with a mean size of 59–308 nm suitable for pulmonary drug delivery were prepared by the ethanol injection method using nickel microengineered flat disc membranes with a uniform pore size of 5–40 μm and a pore spacing of 80 or 200 μm. An ethanolic phase containing 20–50 mg ml−1 phospholipid (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) or Lipoid® E80), 5–12.5 mg ml−1 stabilizer (cholesterol, stearic acid or cocoa butter), and 0 or 5 mg ml−1 vitamin E was injected through the membrane into an agitated aqueous phase at a controlled flux of 142–355 l m−2 h−1 and a shear stress on the membrane surface of 0.80–16 Pa. The mean particle size obtained under optimal conditions was 84 and 59 nm for Lipoid E80 and POPC liposomes, respectively. The particle size of the prepared liposomes increased with an increase in the pore size of the membrane and decreased with an increase in the pore spacing. Lipoid E80 liposomes stabilized by cholesterol or stearic acid maintained their initial size within 3 months. A high entrapment efficiency of 99.87% was achieved when Lipoid E80 liposomes were loaded with vitamin E. Transmission electron microscopy images revealed spherical multi-lamellar structure of vesicles. The reproducibility of the developed fabrication method was high.

Graphical abstract: Preparation of liposomes: a novel application of microengineered membranes - investigation of the process parameters and application to the encapsulation of vitamin E

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Publication details

The article was received on 19 Dec 2012, accepted on 04 Feb 2013 and first published on 05 Feb 2013


Article type: Paper
DOI: 10.1039/C3RA23411H
Citation: RSC Adv., 2013,3, 4985-4994
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    Preparation of liposomes: a novel application of microengineered membranes - investigation of the process parameters and application to the encapsulation of vitamin E

    A. Laouini, C. Charcosset, H. Fessi, R.G. Holdich and G.T. Vladisavljević, RSC Adv., 2013, 3, 4985
    DOI: 10.1039/C3RA23411H

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