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Issue 7, 2013
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Advanced in vivo applications of blue light photoreceptors as alternative fluorescent proteins

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Abstract

The ultimate ambition in cell biology, microbiology and biomedicine is to unravel complex physiological and pathophysiological processes within living organisms. To conquer this challenge, fluorescent proteins (FPs) are used as versatile in vivo reporters and biosensors to study gene regulation as well as the synthesis, localization and function of proteins in living cells. The most widely used FPs are the green fluorescent protein (GFP) and its derivatives and relatives. Their use as in vivo reporter proteins, however, is sometimes restricted by different environmental and cellular factors. Consequently, a whole range of alternative, cofactor-dependent reporter proteins have been developed recently. In this perspective, we summarize the advantages and limitations of the novel class of cyan-green fluorescent flavoproteins in comparison to members of the GFP family and discuss some correlated consequences for the use of FPs as in vivo reporters.

Graphical abstract: Advanced in vivo applications of blue light photoreceptors as alternative fluorescent proteins

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Publication details

The article was received on 06 Feb 2013, accepted on 12 Apr 2013 and first published on 12 Apr 2013


Article type: Perspective
DOI: 10.1039/C3PP50040C
Citation: Photochem. Photobiol. Sci., 2013,12, 1125-1134
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    Advanced in vivo applications of blue light photoreceptors as alternative fluorescent proteins

    T. Drepper, T. Gensch and M. Pohl, Photochem. Photobiol. Sci., 2013, 12, 1125
    DOI: 10.1039/C3PP50040C

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