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Issue 47, 2013
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Modified oligodeoxynucleotide primers for reverse-transcription of target RNAs that can discriminate among length variants at the 3′-terminus

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Abstract

To discriminate among miRNA length variants, we synthesized conformationally restricted or unrestricted oligonucleotides containing a cyclohexyl phosphate residue. These oligonucleotides formed duplexes with length-matched complementary miRNAs more tightly than with length variants. The use of one of these modified oligodeoxynucleotides as a reverse transcription primer enabled a novel RT-PCR that can discriminate among miRNA length variants.

Graphical abstract: Modified oligodeoxynucleotide primers for reverse-transcription of target RNAs that can discriminate among length variants at the 3′-terminus

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Publication details

The article was received on 19 Sep 2013, accepted on 02 Oct 2013 and first published on 02 Oct 2013


Article type: Paper
DOI: 10.1039/C3OB41901K
Citation: Org. Biomol. Chem., 2013,11, 8276-8282
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    Modified oligodeoxynucleotide primers for reverse-transcription of target RNAs that can discriminate among length variants at the 3′-terminus

    Y. Iijima, S. Kojima, E. Kodama, S. Kurohagi, T. Kanamori, Y. Masaki, A. Ohkubo, M. Sekine and K. Seio, Org. Biomol. Chem., 2013, 11, 8276
    DOI: 10.1039/C3OB41901K

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