Issue 35, 2013
From the journal:

Organic & Biomolecular Chemistry

Chemical introduction of the green fluorescence: imaging of cysteine cathepsins by an irreversibly locked GFP fluorophore†

Maxim Frizler,a   Ilia V. Yampolsky,b   Mikhail S. Baranov,b   Marit Stirnberga  and  Michael Gütschow*a  

* Corresponding authors

a Pharmaceutical Institute, Pharmaceutical Chemistry I, University of Bonn, An der Immenburg 4, 53121 Bonn, Germany
E-mail: guetschow@uni-bonn.de
Fax: +49 228 732567
Tel: +49 228 732317

b Institute of Bioorganic Chemistry, Russian Academy of Sciences, Miklukho-Maklaya 16/10, 117997 Moscow, Russia

Abstract

An activity-based probe, containing an irreversibly locked GFP-like fluorophore, was synthesized and evaluated as an inhibitor of human cathepsins and, as exemplified with cathepsin K, it proved to be suitable for ex vivo imaging and quantification of cysteine cathepsins by SDS-PAGE.

Graphical abstract: Chemical introduction of the green fluorescence: imaging of cysteine cathepsins by an irreversibly locked GFP fluorophore

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Article information

DOI
https://doi.org/10.1039/C3OB41341A
Article type
Paper
Submitted
30 Jun 2013
Accepted
16 Jul 2013
First published
16 Jul 2013

Org. Biomol. Chem., 2013,11, 5913-5921

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Chemical introduction of the green fluorescence: imaging of cysteine cathepsins by an irreversibly locked GFP fluorophore

M. Frizler, I. V. Yampolsky, M. S. Baranov, M. Stirnberg and M. Gütschow, Org. Biomol. Chem., 2013, 11, 5913 DOI: 10.1039/C3OB41341A

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