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Issue 43, 2013
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Parallel synthesis and splicing redirection activity of cell-penetrating peptide conjugate libraries of a PNA cargo

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Abstract

A novel method for the parallel synthesis of peptide-biocargo conjugates was developed that utilizes affinity purification for fast isolation of the conjugates in order to avoid time consuming HPLC purification. The methodology was applied to create two libraries of cell-penetrating peptide (CPP)–PNA705 conjugates from parallel-synthesized peptide libraries. The conjugates were tested for their ability to induce splicing redirection in HeLa pLuc705 cells. The results demonstrate how the novel methodology can be applied for screening purposes in order to find suitable CPP-biocargo combinations and further optimization of CPPs.

Graphical abstract: Parallel synthesis and splicing redirection activity of cell-penetrating peptide conjugate libraries of a PNA cargo

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Publication details

The article was received on 14 Aug 2013, accepted on 01 Oct 2013 and first published on 01 Oct 2013


Article type: Paper
DOI: 10.1039/C3OB41659C
Citation: Org. Biomol. Chem., 2013,11, 7621-7630
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    Parallel synthesis and splicing redirection activity of cell-penetrating peptide conjugate libraries of a PNA cargo

    P. J. Deuss, A. A. Arzumanov, D. L. Williams and M. J. Gait, Org. Biomol. Chem., 2013, 11, 7621
    DOI: 10.1039/C3OB41659C

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