Jump to main content
Jump to site search

Issue 16, 2013
Previous Article Next Article

Thiol–yne radical reaction mediated site-specific protein labeling via genetic incorporation of an alkynyl-L-lysine analogue

Author affiliations

Abstract

Three alkyne-containing pyrrolysine derivatives were synthesized and genetically encoded into proteins by a mutant PylRS–tRNA pair with high efficiencies. With these alkyne handles, site-specific dual labeling of proteins can be achieved via a bioorthogonal thiol–yne ligation reaction.

Graphical abstract: Thiol–yne radical reaction mediated site-specific protein labeling via genetic incorporation of an alkynyl-l-lysine analogue

Back to tab navigation

Supplementary files

Publication details

The article was received on 30 Oct 2012, accepted on 31 Jan 2013 and first published on 01 Feb 2013


Article type: Paper
DOI: 10.1039/C3OB27116A
Citation: Org. Biomol. Chem., 2013,11, 2624-2629
  •   Request permissions

    Thiol–yne radical reaction mediated site-specific protein labeling via genetic incorporation of an alkynyl-L-lysine analogue

    Y. Li, M. Pan, Y. Li, Y. Huang and Q. Guo, Org. Biomol. Chem., 2013, 11, 2624
    DOI: 10.1039/C3OB27116A

Search articles by author

Spotlight

Advertisements