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Issue 20, 2013
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Slow translocation of polynucleotides and their discrimination by α-hemolysin inside a single track-etched nanopore designed by atomic layer deposition

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Abstract

We report the formation of a hybrid biological/artificial nanopore by the direct insertion of non-modified α-hemolysin at the entrance of a high aspect ratio (length/diameter) biomimetic nanopore. In this robust hybrid system, the protein exhibits the same polynucleotide discrimination properties as in the biological membrane and the polynucleotide dwell time is strongly increased. This nanopore is very promising for DNA sequencing applications where the high DNA translocation velocity and the fragility of the support are the main bottlenecks.

Graphical abstract: Slow translocation of polynucleotides and their discrimination by α-hemolysin inside a single track-etched nanopore designed by atomic layer deposition

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Publication details

The article was received on 17 Jul 2013, accepted on 06 Aug 2013 and first published on 14 Aug 2013


Article type: Communication
DOI: 10.1039/C3NR03683A
Citation: Nanoscale, 2013,5, 9582-9586
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    Slow translocation of polynucleotides and their discrimination by α-hemolysin inside a single track-etched nanopore designed by atomic layer deposition

    S. Cabello-Aguilar, S. Balme, A. A. Chaaya, M. Bechelany, E. Balanzat, J. Janot, C. Pochat-Bohatier, P. Miele and P. Dejardin, Nanoscale, 2013, 5, 9582
    DOI: 10.1039/C3NR03683A

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