Issue 20, 2013
From the journal:

Nanoscale

Slow translocation of polynucleotides and their discrimination by α-hemolysin inside a single track-etched nanopore designed by atomic layer deposition†

Simon Cabello-Aguilar,a   Sébastien Balme,*ab   Adib Abou Chaaya,a   Mikhael Bechelany,a   Emmanuel Balanzat,c   Jean-Marc Janot,a   Celine Pochat-Bohatier,a   Philippe Mielea  and  Philippe Dejardina  

* Corresponding authors

a Institut Européen des Membranes, UMR5635 CNRS-UM2-ENSCM, Place Eugène Bataillon, 34095 Montpellier Cedex 5, France
E-mail: sebastien.balme@univ-montp2.fr

b Institut Charles Gerhardt UMR 5253 CNRS-UM2-ENSCM-UM1 Université Montpellier 2, Place Eugène Bataillon, 34095 Montpellier Cedex 5, France

c Centre de recherche sur les Ions, les Matériaux et la Photonique, UMR6252 CEA-CNRS-ENSICAEN, 6 Boulevard du Maréchal Juin, 14050 Caen Cedex 4, France

Abstract

We report the formation of a hybrid biological/artificial nanopore by the direct insertion of non-modified α-hemolysin at the entrance of a high aspect ratio (length/diameter) biomimetic nanopore. In this robust hybrid system, the protein exhibits the same polynucleotide discrimination properties as in the biological membrane and the polynucleotide dwell time is strongly increased. This nanopore is very promising for DNA sequencing applications where the high DNA translocation velocity and the fragility of the support are the main bottlenecks.

Graphical abstract: Slow translocation of polynucleotides and their discrimination by α-hemolysin inside a single track-etched nanopore designed by atomic layer deposition

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Article information

DOI
https://doi.org/10.1039/C3NR03683A
Article type
Communication
Submitted
17 Jul 2013
Accepted
06 Aug 2013
First published
14 Aug 2013

Nanoscale, 2013,5, 9582-9586

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Slow translocation of polynucleotides and their discrimination by α-hemolysin inside a single track-etched nanopore designed by atomic layer deposition

S. Cabello-Aguilar, S. Balme, A. A. Chaaya, M. Bechelany, E. Balanzat, J. Janot, C. Pochat-Bohatier, P. Miele and P. Dejardin, Nanoscale, 2013, 5, 9582 DOI: 10.1039/C3NR03683A

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