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Issue 20, 2013
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Impairments of cells and genomic DNA by environmentally transformed engineered nanomaterials

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Abstract

Enormous increase in the production of nanomaterials and their growing applications in the device technology, biotechnology and biomedical areas suggest the need for developing models for predicting the environmental health and safety (EHS) risks posed by such nanomaterials. We hypothesize that CdSe quantum dots (QDs) and ZnO nanoparticles (NPs) encompassed in liposomes or not and transformed by simulated solar UV light can be model systems for studying the environmental toxicity of engineered nanomaterials. In this study, human lung epithelial adenocarcinoma cells (H1650) are exposed to photoirradiated CdSe QDs or ZnO nanopowder included or not in liposomes. The release of cadmium and zinc ions from the nanomaterials exposed to solar simulated UV radiation is detected and quantified by measuring the steady-state and time resolved fluorescence of the metal ion sensor tetracarboxyphenylporphyrin (TCPP) or the commercial Measure iT Pd/Cd sensor. Viability of cells treated with nanomaterials exposed to solar simulated UV radiation for different durations is measured by MTT assay. Enhanced etching of the nanoparticles exposed to solar simulated UV radiation results in the release of toxic levels of heavy metal ions, which considerably lower the viability of H1650 cells is due to the deactivation of DNA repair enzymes as evidenced by the pinching off of nuclear DNA in comet assays and DNA samples in electrophoresis. Results from this study highlight the need to obtain not only quantitative information about the environmental risks posed by engineered nanomaterials but also environment friendly nanomaterials for practical applications.

Graphical abstract: Impairments of cells and genomic DNA by environmentally transformed engineered nanomaterials

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Publication details

The article was received on 17 Jun 2013, accepted on 02 Jul 2013 and first published on 10 Jul 2013


Article type: Communication
DOI: 10.1039/C3NR03118G
Citation: Nanoscale, 2013,5, 9511-9516
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    Impairments of cells and genomic DNA by environmentally transformed engineered nanomaterials

    P. Jones, S. Sugino, S. Yamamura, F. Lacy and V. Biju, Nanoscale, 2013, 5, 9511
    DOI: 10.1039/C3NR03118G

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