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Issue 19, 2013
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Peptide-based carbon nanotubes for mitochondrial targeting

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Abstract

In the present study, we report the design and synthesis of peptide-based-multi-walled carbon nanotubes (MWCNTs) to target mitochondria. Targeting these intracellular organelles might open the way to develop alternative systems to address diseases related to genetic mutations in mitochondrial (mt)-DNA, by delivering therapeutic oligonucleotides. The first step towards mitochondrial delivery of this type of nucleic acid was to target MWCNTs to mitochondria by covalent functionalization with a well-known endogenous mitochondrial targeting sequence (MTS). The subcellular localization of the conjugates, which were fluorescently labeled, in murine RAW 264.7 macrophages and human HeLa cells was then studied using different microscopy techniques, such as wide-field epifluorescence microscopy, confocal laser scanning microscopy (CLSM) and transmission electron microscopy (TEM). The localization of the MTS–MWCNT conjugates into mitochondria was further confirmed by analyzing the isolated organelles using TEM.

Graphical abstract: Peptide-based carbon nanotubes for mitochondrial targeting

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Publication details

The article was received on 24 May 2013, accepted on 04 Jul 2013 and first published on 11 Jul 2013


Article type: Paper
DOI: 10.1039/C3NR02694A
Citation: Nanoscale, 2013,5, 9110-9117
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    Peptide-based carbon nanotubes for mitochondrial targeting

    A. Battigelli, J. Russier, E. Venturelli, C. Fabbro, V. Petronilli, P. Bernardi, T. Da Ros, M. Prato and A. Bianco, Nanoscale, 2013, 5, 9110
    DOI: 10.1039/C3NR02694A

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