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Issue 13, 2013
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Label-free electrochemical impedance genosensor based on 1-aminopyrene/graphene hybrids

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Abstract

In this work, we proposed a novel simple protocol for preparing 1-aminopyrene/graphene (ApG) hybrids for fabricating label-free electrochemical impedance genosensor. Graphene, with the structure of a single-atom-thick sheet of sp2-bonded carbon atoms, was anchored to 1-aminopyrene (1-Ap) with the pyrenyl group via π-stacking interaction. The morphology, conductivity, and interaction of ApG hybrids were characterized by transmission electron microscopy (TEM), cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), UV-visible (UV-vis) and fluorescence spectra. The amino-substituted oligonucleotide probe was conjugated to 1-Ap by the cross-linker glutaraldehyde. The DNA hybridization reaction of oligonucleotide probe with target DNA was monitored by EIS. Under optimum conditions, the proposed biosensor exhibited high sensitivity and a low detection limit for detecting the complementary oligonucleotide. The target oligonucleotide could be quantified in a wide range of 1.0 × 10−12 to 1.0 × 10−8 M with good linearity (R = 0.9900) and low detection limit of 4.5 × 10−13 M (S/N = 3).

Graphical abstract: Label-free electrochemical impedance genosensor based on 1-aminopyrene/graphene hybrids

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Publication details

The article was received on 11 Mar 2013, accepted on 15 Apr 2013 and first published on 22 Apr 2013


Article type: Paper
DOI: 10.1039/C3NR01237A
Citation: Nanoscale, 2013,5, 5833-5840
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    Label-free electrochemical impedance genosensor based on 1-aminopyrene/graphene hybrids

    L. Luo, Z. Zhang, Y. Ding, D. Deng, X. Zhu and Z. Wang, Nanoscale, 2013, 5, 5833
    DOI: 10.1039/C3NR01237A

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