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Issue 14, 2013
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Singlet oxygen plays a key role in the toxicity and DNA damage caused by nanometric TiO2 in human keratinocytes

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Abstract

Nanometric TiO2 has been reported to be cytotoxic and genotoxic in different in vitro models when activated by UV light. However, a clear picture of the species mediating the observed toxic effects is still missing. Here, a nanometric TiO2 powder has been modified at the surface to completely inhibit its photo-catalytic activity and to inhibit the generation of all reactive species except for singlet oxygen. The prepared powders have been tested for their ability to induce strand breaks in plasmid DNA and for their cytotoxicity and genotoxicity toward human keratinocyte (HaCaT) cells (100–500 μg mL−1, 15 min UVA/B exposure at 216–36 mJ m−2 respectively). The data reported herein indicate that the photo-toxicity of TiO2 is mainly triggered by particle-derived singlet oxygen. The data presented herein contribute to the knowledge of structure–activity relationships which are needed for the design of safe nanomaterials.

Graphical abstract: Singlet oxygen plays a key role in the toxicity and DNA damage caused by nanometric TiO2 in human keratinocytes

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Publication details

The article was received on 11 Mar 2013, accepted on 08 May 2013 and first published on 09 May 2013


Article type: Paper
DOI: 10.1039/C3NR01191G
Citation: Nanoscale, 2013,5, 6567-6576
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    Singlet oxygen plays a key role in the toxicity and DNA damage caused by nanometric TiO2 in human keratinocytes

    I. Fenoglio, J. Ponti, E. Alloa, M. Ghiazza, I. Corazzari, R. Capomaccio, D. Rembges, S. Oliaro-Bosso and F. Rossi, Nanoscale, 2013, 5, 6567
    DOI: 10.1039/C3NR01191G

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