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Issue 3, 2013
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Near-infrared fluorescent ribonuclease-A-encapsulated gold nanoclusters: preparation, characterization, cancer targeting and imaging

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Abstract

Ultra-small gold nanoclusters (AuNCs) have unique size-dependent optical, electrical and chemical properties. They have emerged as a new nanomaterial with broad applications in optoelectronics, catalysis, biosensing, and bioimaging. Several strategies have been exploited to prepare AuNCs of different “magic number” sizes, using different templates e.g. dendrimers, polyethyleneimines, peptides, and more recently, proteins. Notwithstanding, almost all bio-template-protected AuNCs reported so far exhibit fairly low fluorescence quantum yields (QYs), typically <5%, which is especially true for AuNCs prepared using the protein templates. In this paper, we report a facile, one-pot aqueous synthesis of highly fluorescent AuNCs using bovine pancreatic ribonuclease A (RNase-A) as the bio-template. The as-prepared AuNCs not only fluoresce strongly at the near-infrared (NIR) region (λem = 682 nm), but also exhibit an elevated QY of ∼12%. Additionally, the RNase-A-encapsulated AuNC (RNase-A-AuNC) displays an exceptionally large Stokes shift of ∼210 nm as well as a single dominant fluorescence lifetime of ∼1.5 μs, about three orders of magnitude longer than biological autofluorescence. Furthermore, by coupling vitamin B12 (VB12) to the RNase-A-AuNC, we develop a multifunctional nanoplatform that is suitable for simultaneous targeting and imaging of cancer at the cellular level using Caco-2 cell lines as an in vitro model. Since VB12 has effective uptake pathways in the digestive system, this nanoplatform may have potential for targeted oral drug delivery in vivo.

Graphical abstract: Near-infrared fluorescent ribonuclease-A-encapsulated gold nanoclusters: preparation, characterization, cancer targeting and imaging

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Publication details

The article was received on 19 Aug 2012, accepted on 26 Nov 2012 and first published on 27 Nov 2012


Article type: Paper
DOI: 10.1039/C2NR32760K
Citation: Nanoscale, 2013,5, 1009-1017
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    Near-infrared fluorescent ribonuclease-A-encapsulated gold nanoclusters: preparation, characterization, cancer targeting and imaging

    Y. Kong, J. Chen, F. Gao, R. Brydson, B. Johnson, G. Heath, Y. Zhang, L. Wu and D. Zhou, Nanoscale, 2013, 5, 1009
    DOI: 10.1039/C2NR32760K

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