Jump to main content
Jump to site search

Issue 5, 2013
Previous Article Next Article

Suppression of ZIP8 expression is a common feature of cadmium-resistant and manganese-resistant RBL-2H3 cells

Author affiliations

Abstract

Rat basophilic leukemia RBL-2H3 cells show markedly high sensitivity to both CdCl2 and MnCl2 compared with other rat cell lines, due to efficient accumulation of cadmium and manganese. To clarify the roles of metal transporters in hyperaccumulation of cadmium and manganese in RBL-2H3 cells, Cd-resistant and Mn-resistant cells were developed from RBL-2H3 cells by continuous exposure to CdCl2 and MnCl2, respectively. The established Cd-resistant (RBL-Cdr) and Mn-resistant (RBL-Mnr) cells exhibited about 20 times higher LC50 values of CdCl2 and MnCl2, respectively, than parental RBL-2H3 cells, and showed cross-resistance to each metal. The resistance to cadmium and manganese was primarily conferred by a marked decrease in the uptake of both metals. RBL-Cdr cells also showed cross-resistance to HgCl2 and AgNO3 probably due to enhanced expression of metallothionein. Among the possible transporters involved in the uptake of Cd2+ and Mn2+, the expression of ZIP8 (Zrt-, Irt-related protein 8), encoded by Slc39a8, showed a marked suppression in both RBL-Cdr and RBL-Mnr cells. These results suggest that ZIP8 plays a pivotal role in the transport and toxicity of Cd2+ and Mn2+ in RBL-2H3 cells.

Graphical abstract: Suppression of ZIP8 expression is a common feature of cadmium-resistant and manganese-resistant RBL-2H3 cells

Back to tab navigation

Supplementary files

Publication details

The article was received on 02 Jan 2013, accepted on 05 Apr 2013 and first published on 08 Apr 2013


Article type: Paper
DOI: 10.1039/C3MT00003F
Citation: Metallomics, 2013,5, 437-444
  •   Request permissions

    Suppression of ZIP8 expression is a common feature of cadmium-resistant and manganese-resistant RBL-2H3 cells

    H. Fujishiro, T. Ohashi, M. Takuma and S. Himeno, Metallomics, 2013, 5, 437
    DOI: 10.1039/C3MT00003F

Search articles by author

Spotlight

Advertisements