Jump to main content
Jump to site search

Issue 12, 2013
Previous Article Next Article

Activity-based protein profiling of secreted cellulolytic enzyme activity dynamics in Trichoderma reesei QM6a, NG14, and RUT-C30

Author affiliations

Abstract

Lignocellulosic biomass has great promise as a highly abundant and renewable source for the production of biofuels. However, the recalcitrant nature of lignocellulose toward hydrolysis into soluble sugars remains a significant challenge to harnessing the potential of this source of bioenergy. A primary method for deconstructing lignocellulose is via chemical treatments, high temperatures, and hydrolytic enzyme cocktails, many of which are derived from the fungus Trichoderma reesei. Herein, we use an activity-based probe for glycoside hydrolases to rapidly identify optimal conditions for maximum enzymatic lignocellulose deconstruction. We also demonstrate that subtle changes to enzyme composition and activity in various strains of T. reesei can be readily characterized by our probe approach. The approach also permits multimodal measurements, including fluorescent gel-based analysis of activity in response to varied conditions and treatments, and mass spectrometry-based quantitative identification of labelled proteins. We demonstrate the promise this probe approach holds to facilitate rapid production of enzyme cocktails for high-efficiency lignocellulose deconstruction to accommodate high-yield biofuel production.

Graphical abstract: Activity-based protein profiling of secreted cellulolytic enzyme activity dynamics in Trichoderma reesei QM6a, NG14, and RUT-C30

Back to tab navigation

Supplementary files

Publication details

The article was received on 05 Aug 2013, accepted on 07 Oct 2013 and first published on 09 Oct 2013


Article type: Paper
DOI: 10.1039/C3MB70333A
Citation: Mol. BioSyst., 2013,9, 2992-3000
  •   Request permissions

    Activity-based protein profiling of secreted cellulolytic enzyme activity dynamics in Trichoderma reesei QM6a, NG14, and RUT-C30

    L. N. Anderson, D. E. Culley, B. A. Hofstad, L. M. Chauvigné-Hines, E. M. Zink, S. O. Purvine, R. D. Smith, S. J. Callister, J. M. Magnuson and A. T. Wright, Mol. BioSyst., 2013, 9, 2992
    DOI: 10.1039/C3MB70333A

Search articles by author

Spotlight

Advertisements