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Issue 5, 2013
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Fluorescent peptide indicator displacement assay for monitoring interactions between RNA and RNA binding proteins

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Abstract

This paper describes a sensitive, non-destructive displacement assay, using a fluorescent peptide indicator, for real-time monitoring of the interactions between RNA and RNA binding proteins (RBPs). The developed fluorescent peptide indicators, each containing a mid-sequence fluorophore unit, allowed sensing of target RNA and RNA–RBP interactions through changes in fluorescence intensity. We anticipate that this assay will open up new possibilities for meaningful studies of RNA–RBP interactions.

Graphical abstract: Fluorescent peptide indicator displacement assay for monitoring interactions between RNA and RNA binding proteins

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Publication details

The article was received on 24 Oct 2012, accepted on 11 Dec 2012 and first published on 13 Dec 2012


Article type: Communication
DOI: 10.1039/C2MB25470K
Citation: Mol. BioSyst., 2013,9, 948-951
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    Fluorescent peptide indicator displacement assay for monitoring interactions between RNA and RNA binding proteins

    H. S. Jeong, S. M. Choi, H. W. Kim, J. W. Park, H. N. Park, S. M. Park, S. K. Jang, Y. M. Rhee and B. H. Kim, Mol. BioSyst., 2013, 9, 948
    DOI: 10.1039/C2MB25470K

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