Cationic cholesterol-modified gelatin as an in vitro siRNA delivery vehicle

Abstract

Polymeric micelle prepared through the self-assembly of cationic cholesterol-modified gelatin was tested for siRNA delivery. It exerted the desired effect of gene knockdown in HeLa cells stably expressing the luciferase gene and achieved a two-fold increase in the knockdown ability when compared to Lipofectamine® 2000. It was found that the polymeric micelle exhibited excellent stability and increased the biological stability of the siRNA in serum.