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Issue 23, 2013
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Digital holography as a method for 3D imaging and estimating the biovolume of motile cells

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Abstract

Sperm morphology is regarded as a significant prognostic factor for fertilization, as abnormal sperm structure is one of the most common factors in male infertility. Furthermore, obtaining accurate morphological information is an important issue with strong implications in zoo-technical industries, for example to perform sorting of species X from species Y. A challenging step forward would be the availability of a fast, high-throughput and label-free system for the measurement of physical parameters and visualization of the 3D shape of such biological specimens. Here we show a quantitative imaging approach to estimate simply and quickly the biovolume of sperm cells, combining the optical tweezers technique with digital holography, in a single and integrated set-up for a biotechnology assay process on the lab-on-a-chip scale. This approach can open the way for fast and high-throughput analysis in label-free microfluidic based “cytofluorimeters” and prognostic examination based on sperm morphology, thus allowing advancements in reproductive science.

Graphical abstract: Digital holography as a method for 3D imaging and estimating the biovolume of motile cells

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Publication details

The article was received on 23 Apr 2013, accepted on 12 Sep 2013 and first published on 12 Sep 2013


Article type: Technical Innovation
DOI: 10.1039/C3LC50515D
Citation: Lab Chip, 2013,13, 4512-4516
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    Digital holography as a method for 3D imaging and estimating the biovolume of motile cells

    F. Merola, L. Miccio, P. Memmolo, G. Di Caprio, A. Galli, R. Puglisi, D. Balduzzi, G. Coppola, P. Netti and P. Ferraro, Lab Chip, 2013, 13, 4512
    DOI: 10.1039/C3LC50515D

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